TY  - JOUR
A1  - Scherr, Dietmar
A1  - Jeck, Reinhard
A1  - Berghäuser, Jürgen
A1  - Woenckhaus, Christoph
T1  - Komplexbildung von Dehydrogenasen mit Coenzymanalogen und Coenzymbruchstücken
T1  - Complexformation of dehydrogenases with coenzyme analogues and coenzyme fragments
T2  - Zeitschrift für Naturforschung, C
N2  - The spectral properties of binary complexes of NAD-analogues and fragments therefrom with I.DH from pig heart or ADH from liver and yeast have been investigated. The NADH-analogues were modified by replacing adenine through benzimidazole, benzene or dihydronicotinamide. Additionally adenosine diphosphate ribose, dihydronicotinamide and dihydronicotinamide- ribose pyrophosphate-5"-ribose have been studied.
It has been shown by means of difference spectra that complexes between ADH from horse liver and analogues cause spectral changes in the region of aromatic absorption at 280 nm even when adenine is absent in the analogues. Spectral changes in the other enzymes mentioned are probably due to changes of the n-π* absorption of the adenine ring. The spectral changes upon complexing indicate hydrophobic interaction of the adenine with the enzyme protein. Fluorescence spectra vary in the intensity of the energy transfer band as well as in coenzyme emission depending on variation of the coenzym analogue. Changing of complex formation between protein and analogues at different pH-values are investigated. ADH from yeast, especially, shows a pK around 6 which suggests interaction with histidine imidazole.
KW  - Coenzyme analogues
KW  - dehydrogenases
KW  - difference spectra
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/79961
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-799616
SN  - 1865-7125
SN  - 0939-5075
VL  - 28.1973
IS  - 5-6
SP  - 247
EP  - 254
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -