TY  - JOUR
A1  - Casanas, Arnau
A1  - Warshamanage, Rangana
A1  - Finke, Aaron D.
A1  - Panepucci, Ezequiel
A1  - Olieric, Vincent
A1  - Nöll, Anne
A1  - Tampé, Robert
A1  - Brandstetter, Stefan
A1  - Förster, Andreas
A1  - Mueller, Marcus
A1  - Schulze-Briese, Clemens
A1  - Bunk, Oliver
A1  - Wang, Meitian
T1  - EIGER detector : application in macromolecular crystallography
T2  - Acta crystallographica. Section D, Biological crystallography online
N2  - The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 × 75 µm, frame rates of up to 3000 Hz and a dead time as low as 3.8 µs. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine φ-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed.
KW  - X-ray detectors
KW  - EIGER detector
KW  - macromolecular crystallography
KW  - data-collection strategy
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/42885
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-428850
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013597
SN  - 2059-7983
SN  - 1399-0047
SN  - 0907-4449
N1  - This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
VL  - 72
IS  - Pt 9
SP  - 1036
EP  - 1048
PB  - Wiley-Blackwell
CY  - Oxford
ER  -