TY  - INPR
A1  - Coppin, Emilie
A1  - Sundarasetty, Bala Sai
A1  - Rahmig, Susann
A1  - Blume, Jonas
A1  - Verheyden, Nikita A.
A1  - Bahlmann, Franz
A1  - Ravens, Sarina
A1  - Schubert, Undine
A1  - Schmid, Janine
A1  - Ludwig, Stefan
A1  - Kaisenberg, Constantin von
A1  - Platz, Alexander
A1  - Naumann, Ronald
A1  - Ludwig, Barbara
A1  - Prinz, Immo
A1  - Waskow, Claudia
A1  - Krueger, Andreas
T1  - Enhanced differentiation of functional human T cells in NSGW41 mice with tissue-specific expression of human interleukin-7
T2  - bioRxiv
N2  - Humanized mouse models have become increasingly valuable tools to study human hematopoiesis and infectious diseases. However, human T cell differentiation remains inefficient. We generated mice expressing human interleukin (IL-7), a critical growth and survival factor for T cells, under the control of murine IL-7 regulatory elements. After transfer of human cord blood-derived hematopoietic stem and progenitor cells, transgenic mice on the NSGW41 background, termed NSGW41hIL7, showed elevated and prolonged human cellularity in the thymus while maintaining physiological ratios of thymocyte subsets. As a consequence, numbers of functional human T cells in the periphery were increased without evidence for pathological lymphoproliferation or aberrant expansion of effector or memory-like T cells. We conclude that the novel NSGW41hIL7 strain represents an optimized mouse model for humanization to better understand human T cell differentiation in vivo and to generate a human immune system with a better approximation of human lymphocyte ratios.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72733
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-727337
IS  - 2020.04.24.060319
ER  -