TY  - JOUR
A1  - Richter-Heitmann, Tim
A1  - Hofner, Benjamin
A1  - Krah, Franz-Sebastian
A1  - Sikorski, Johannes
A1  - Wüst, Pia K.
A1  - Bunk, Boyke
A1  - Huang, Sixing
A1  - Regan, Kathleen M.
A1  - Berner, Doreen
A1  - Boeddinghaus, Runa S.
A1  - Marhan, Sven
A1  - Prati, Daniel
A1  - Kandeler, Ellen
A1  - Overmann, Jörg
A1  - Friedrich, Michael W.
T1  - Stochastic dispersal rather than deterministic selection explains the spatio-temporal distribution of soil bacteria in a temperate grassland
T2  - Frontiers in microbiology
N2  - Spatial and temporal processes shaping microbial communities are inseparably linked but rarely studied together. By Illumina 16S rRNA sequencing, we monitored soil bacteria in 360 stations on a 100 square meter plot distributed across six intra-annual samplings in a rarely managed, temperate grassland. Using a multi-tiered approach, we tested the extent to which stochastic or deterministic processes influenced the composition of local communities. A combination of phylogenetic turnover analysis and null modeling demonstrated that either homogenization by unlimited stochastic dispersal or scenarios, in which neither stochastic processes nor deterministic forces dominated, explained local assembly processes. Thus, the majority of all sampled communities (82%) was rather homogeneous with no significant changes in abundance-weighted composition. However, we detected strong and uniform taxonomic shifts within just nine samples in early summer. Thus, community snapshots sampled from single points in time or space do not necessarily reflect a representative community state. The potential for change despite the overall homogeneity was further demonstrated when the focus shifted to the rare biosphere. Rare OTU turnover, rather than nestedness, characterized abundance-independent β-diversity. Accordingly, boosted generalized additive models encompassing spatial, temporal and environmental variables revealed strong and highly diverse effects of space on OTU abundance, even within the same genus. This pure spatial effect increased with decreasing OTU abundance and frequency, whereas soil moisture – the most important environmental variable – had an opposite effect by impacting abundant OTUs more than the rare ones. These results indicate that – despite considerable oscillation in space and time – the abundant and resident OTUs provide a community backbone that supports much higher β-diversity of a dynamic rare biosphere. Our findings reveal complex interactions among space, time, and environmental filters within bacterial communities in a long-established temperate grassland.
KW  - spatio-temporal analysis
KW  - soil bacteria communities
KW  - community assembly
KW  - variable selection
KW  - generalized additive model
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/55039
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-550394
SN  - 1664-302X
VL  - 11
IS  - 1391
PB  - Frontiers Media
CY  - Lausanne
ER  -