TY  - JOUR
A1  - Udeabor, Samuel Ebele
A1  - Herrera-Vizcaíno, Carlos
A1  - Sader, Robert Alexander
A1  - Kirkpatrick, James
A1  - Maawi, Sarah al-
A1  - Ghanaati, Shahram Michael
T1  - Characterization of the cellular reaction to a collagen-based matrix: an in vivo histological and histomorphometrical analysis
T2  - Materials
N2  - The permeability and inflammatory tissue reaction to Mucomaix® matrix (MM), a non- cross-linked collagen-based matrix was evaluated in both ex vivo and in vivo settings. Liquid platelet rich fibrin (PRF), a blood concentrate system, was used to assess its capacity to absorb human proteins and interact with blood cells ex vivo. In the in vivo aspect, 12 Wister rats had MM implanted subcutaneously, whereas another 12 rats (control) were sham-operated without biomaterial implantation. On days 3, 15 and 30, explantation was completed (four rats per time-point) to evaluate the tissue reactions to the matrix. Data collected were statistically analyzed using analysis of variance (ANOVA) and Tukey multiple comparisons tests (GraphPad Prism 8). The matrix absorbed the liquid PRF in the ex vivo study. Day 3 post-implantation revealed mild tissue inflammatory reaction with presence of mononuclear cells in the implantation site and on the biomaterial surface (mostly CD68-positive macrophages). The control group at this stage had more mononuclear cells than the test group. From day 15, multinucleated giant cells (MNGCs) were seen in the implantation site and the outer third of the matrix with marked increase on day 30 and spread to the matrix core. The presence of these CD68-positive MNGCs was associated with significant matrix vascularization. The matrix degraded significantly over the study period, but its core was still visible as of day 30 post-implantation. The high permeability and fast degradation properties of MM were highlighted.
KW  - cellular reaction
KW  - collagen-based matrix
KW  - Mucomaix® matrix
KW  - histology
KW  - histomorphometry
KW  - liquid PRF
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54719
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-547199
SN  - 1996-1944
VL  - 13
IS  - 2730
PB  - MDPI
CY  - Basel
ER  -