TY  - JOUR
A1  - Siebenbrodt, Kai
A1  - Schütz, Vanessa
A1  - Costard, Lara Sophie
A1  - Neubert, Valentin
A1  - Alvarez-Fischer, Daniel
A1  - Seidel, Kerstin
A1  - Schmeck, Bernd
A1  - Meuth, Sven
A1  - Rosenow, Felix
A1  - Bauer, Sebastian
T1  - Hippocampal cytokine release in experimental epileptogenesis - a longitudinal in vivo microdialysis study
T2  - Brain Sciences
N2  - Background: Inflammation, particularly cytokine release, contributes to epileptogenesis by influencing the cerebral tissue remodeling and neuronal excitability that occurs after a precipitating epileptogenic insult. While several cytokines have been explored in this process, release kinetics are less well investigated. Determining the time course of cytokine release in the epileptogenic zone is necessary for precisely timed preventive or therapeutic anti-inflammatory interventions. Methods: Hippocampal extracellular levels of six cytokines and chemokines (IL-1β, IL-6, IL-10, CCL2, CCL3, and CCL5) were quantified at various time points during epileptogenesis in a rat model of mesial temporal lobe epilepsy with hippocampal sclerosis (mTLE-HS) using microdialysis (MD). Results: The analysis of microdialysates demonstrated consistent elevation at all time points during epileptogenesis for IL-1β and IL-10. IL-10 release was maximal on day 1, IL-1β release peaked at day 8. No correlation between local hippocampal IL-1β concentrations and IL-1β blood levels was found. Conclusion: The release kinetics of IL-1β are consistent with its established pro-epileptogenic properties, while the kinetics of IL-10 suggest a counter-regulatory effect. This proof-of-concept study demonstrates the feasibility of intraindividual longitudinal monitoring of hippocampal molecular inflammatory processes via repetitive MD over several weeks and sheds light on the kinetics of hippocampal cytokine release during epileptogenesis.
KW  - epilepsy
KW  - temporal lobe epilepsy
KW  - inflammation
KW  - hippocampal sclerosis
KW  - rat model
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/82374
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-823748
SN  - 2076-3425
N1  - The authors were supported by the LOEWE Center for Personalized Translational Epilepsy Research (CePTER), Goethe University, Frankfurt am Main, Germany, funded by the Hessen State Ministry for Higher Education, Research and the Arts (HMWK).
VL  - 12
IS  - 5, art. 677
SP  - 1
EP  - 12
PB  - MDPI
CY  - Basel
ER  -