TY  - INPR
A1  - Waard, Antonius A. de
A1  - Verkerk, Tamara
A1  - Jongsma, Marlieke L. M.
A1  - Hoefakker, Kelly
A1  - Sethumadhavan, Sunesh
A1  - Gerke, Carolin
A1  - Bliss, Sophie
A1  - Janssen, George M. C.
A1  - Ru, Arnoud H. de
A1  - Claas, Frans H. J.
A1  - Mulder, Arend
A1  - Tampé, Robert
A1  - Veelen, Peter A. van
A1  - Halenius, Anne
A1  - Spaapen, Robbert M.
T1  - PAKC: a novel panel of HLA class I antigen presentation machinery knockout cells from the same genetic origin
T2  - bioRxiv
N2  - With the emergence of immunotherapies, the understanding of functional HLA class I antigen presentation to T cells is more relevant than ever. Current knowledge on antigen presentation is based on decades of research in a wide variety of cell types with varying antigen presentation machinery (APM) expression patterns, proteomes and HLA haplotypes. This diversity complicates the establishment of individual APM contributions to antigen generation, selection and presentation. Therefore, we generated a novel Panel of APM Knockout Cell lines (PAKC) from the same genetic origin. After CRISPR/Cas9 genome-editing of ten individual APM components in a human cell line, we derived clonal cell lines and confirmed their knockout status and phenotype. We then show how PAKC will accelerate research on the functional interplay between APM components and their role in antigen generation and presentation. This will lead to improved understanding of peptide-specific T cell responses in infection, cancer and autoimmunity.
KW  - Human
KW  - Antigens/Peptides/Epitopes
KW  - MHC
KW  - HLA class I
KW  - antigen processing and presentation
KW  - CRISPR/Cas9
KW  - knockout
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72681
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-726819
IS  - 2020.01.24.917807
ER  -