TY  - JOUR
A1  - Vögele, Jennifer
A1  - Ferner, Jan
A1  - Altınçekiç, Nadide
A1  - Bains, Jasleen Kaur
A1  - Ceylan, Betül
A1  - Fürtig, Boris
A1  - Grün, J. Tassilo
A1  - Hengesbach, Martin
A1  - Hohmann, Katharina Felicitas
A1  - Hymon, Daniel
A1  - Knežić, Božana
A1  - Löhr, Frank
A1  - Peter, Stephen A.
A1  - Pyper, Dennis Joshua
A1  - Qureshi, Nusrat
A1  - Richter, Christian
A1  - Schlundt, Andreas
A1  - Schwalbe, Harald
A1  - Stirnal, Elke
A1  - Sudakov, Alexey
A1  - Wacker, Anna
A1  - Weigand, Julia
A1  - Wirmer-Bartoschek, Julia
A1  - Wöhnert, Jens
A1  - Duchardt-Ferner, Elke
T1  - 1H, 13C, 15N and 31P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2
T2  - Biomolecular NMR assignments
N2  - The SARS-CoV-2 virus is the cause of the respiratory disease COVID-19. As of today, therapeutic interventions in severe COVID-19 cases are still not available as no effective therapeutics have been developed so far. Despite the ongoing development of a number of effective vaccines, therapeutics to fight the disease once it has been contracted will still be required. Promising targets for the development of antiviral agents against SARS-CoV-2 can be found in the viral RNA genome. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are highly conserved among Betacoronaviruses and contain structured RNA elements involved in the translation and replication of the viral genome. The 40 nucleotides (nt) long highly conserved stem-loop 4 (5_SL4) is located within the 5′-untranslated region (5′-UTR) important for viral replication. 5_SL4 features an extended stem structure disrupted by several pyrimidine mismatches and is capped by a pentaloop. Here, we report extensive 1H, 13C, 15N and 31P resonance assignments of 5_SL4 as the basis for in-depth structural and ligand screening studies by solution NMR spectroscopy.
KW  - SARS-CoV-2
KW  - RNA genome
KW  - 5′-UTR
KW  - 5_SL4
KW  - Solution NMR spectroscopy
KW  - COVID19-NMR
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/63659
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-636594
SN  - 1874-270X
N1  - Work at the Center for Biomolecular Magnetic Resonance (BMRZ) at the Goethe-University Frankfurt is supported by the state of Hesse. Work in Covid19-nmr was supported by the Goethe Corona Funds, by the IWB-EFRE-programme 20007375 of the state of Hesse, and the DFG in CRC902: “Molecular Principles of RNA-based regulation.” and infrastructure funds (Project Numbers: 277478796, 277479031, 392682309, 452632086, 70653611). H.S. and B.F. are supported by the DFG in graduate school CLIC (GRK 1986).
N1  - Open Access funding enabled and organized by Projekt DEAL.
VL  - 15.2021
IS  - 2
SP  - 335
EP  - 340
PB  - Springer Netherlands
CY  - Dordrecht [u.a.]
ER  -