TY  - JOUR
A1  - Pfeiffer, Kathy
A1  - Gohil, Vishal
A1  - Stuart, Rosemary A.
A1  - Hunte, Carola
A1  - Brandt, Ulrich
A1  - Greenberg, Miriam L.
A1  - Schägger, Hermann
T1  - Cardiolipin stabilizes respiratory chain supercomplexes
T2  - Journal of biological chemistry
N2  - Cardiolipin stabilized supercomplexes of Saccharomyces cerevisiae respiratory chain complexes III and IV (ubiquinol:cytochrome c oxidoreductase and cytochrome c oxidase, respectively), but was not essential for their formation in the inner mitochondrial membrane because they were found also in a cardiolipin-deficient strain. Reconstitution with cardiolipin largely restored wild-type stability. The putative interface of complexes III and IV comprises transmembrane helices of cytochromes b and c1 and tightly bound cardiolipin. Subunits Rip1p, Qcr6p, Qcr9p, Qcr10p, Cox8p, Cox12p, and Cox13p and cytochrome c were not essential for the assembly of supercomplexes; and in the absence of Qcr6p, the formation of supercomplexes was even promoted. An additional marked effect of cardiolipin concerns cytochrome c oxidase. We show that a cardiolipin-deficient strain harbored almost inactive resting cytochrome c oxidase in the membrane. Transition to the fully active pulsed state occurred on a minute time scale.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/76040
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-760401
SN  - 0021-9258
VL  - 278.2003
IS  - 52
SP  - 52873
EP  - 52880
PB  - American Society for Biochemistry and Molecular Biology Publications
CY  - Bethesda, Md
ER  -