TY  - JOUR
A1  - Schnieders, Robbin
A1  - Peter, Stephen
A1  - Banijamali, Elnaz
A1  - Riad, Magdalena
A1  - Altınçekiç, Nadide
A1  - Bains, Jasleen Kaur
A1  - Ceylan, Betül
A1  - Fürtig, Boris
A1  - Grün, J. Tassilo
A1  - Hengesbach, Martin
A1  - Hohmann, Katharina Felicitas
A1  - Hymon, Daniel
A1  - Knežić, Božana
A1  - Oxenfarth, Andreas
A1  - Petzold, Katja
A1  - Qureshi, Nusrat
A1  - Richter, Christian
A1  - Schlagnitweit, Judith
A1  - Schlundt, Andreas
A1  - Schwalbe, Harald
A1  - Stirnal, Elke
A1  - Sudakov, Alexey
A1  - Vögele, Jennifer
A1  - Wacker, Anna
A1  - Weigand, Julia
A1  - Wirmer-Bartoschek, Julia
A1  - Wöhnert, Jens
T1  - 1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5′-UTR of SARS-CoV-2
T2  - Biomolecular NMR assignments
N2  - The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5′- and 3′-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5′-untranslated region (5′-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5′-UUUCGU-3′ hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive 1H, 13C and 15N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.
KW  - SARS-CoV-2
KW  - 5′-UTR
KW  - SL5a
KW  - Solution NMR spectroscopy
KW  - COVID19-NMR
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/62837
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-628372
SN  - 1874-270X
N1  - Work at BMRZ is supported by the state of Hesse. Work in Covid19-nmr was supported by the Goethe Corona Funds, by the IWB-EFRE-programme 20007375 of state of Hesse, the DFG in CRC902: “Molecular Principles of RNA-based regulation.” and infrastructure funds (Project Numbers: 277478796, 277479031, 392682309, 452632086, 70653611). Open Access funding enabled and organized by Projekt DEAL.
VL  - 15
IS  - 1
SP  - 203
EP  - 211
PB  - Springer Netherlands
CY  - Dordrecht [u.a.]
ER  -