TY  - JOUR
A1  - Schmidt, Stanislaw
A1  - Luckowitsch, Marie
A1  - Hogardt, Michael
A1  - Lehrnbecher, Thomas
T1  - Natural killer cell line NK-92-mediated damage of medically important fungi
T2  - Journal of Fungi
N2  - Invasive fungal disease (IFD) in hematopoietic stem cell transplantation is associatedwith high morbidity and mortality. As the antifungal host response determines risk and outcomeof IFD, there is growing interest in adoptive immunotherapy using T cells or natural killer (NK)cells. Although the NK-92 cell line has been tested as anticancer therapy in clinical trials, data onthe antifungal activity of NK-92 cells are lacking. Here, we show that the NK-92 cell line exhibitsconsiderable fungal damage on all medically important fungi tested, such as different species ofAspergillus,Candida,  mucormycetes,  andFusarium.   The  extent  of  fungal  damage  differs  acrossvarious species of mucormycetes andFusarium, whereas it is comparable across different species ofAspergillusandCandida. Interferon (IFN)-γlevels in the supernatant were lower when NK-92 cells areco-incubated withAspergillus fumigatus,Candida albicans, orRhizopus arrhizuscompared to the levelswhen NK-92 cells are incubated alone. Different to primary human NK cells, no increase of perforinlevels in the supernatant was observed when the fungi were added to NK-92 cells. Ourin vitrodatademonstrated that the NK-92 cell line could be a feasible tool for antifungal immunotherapy, butdata of animal models are warranted prior to clinical trials.
KW  - natural killer cell
KW  - NK-92
KW  - antifungal activity
KW  - Aspergillus
KW  - Candida
KW  - Fusarium
KW  - mucormycetes
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/61794
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-617942
SN  - 2309-608X
VL  - 7
IS  - 2, art. 144
SP  - 1
EP  - 14
PB  - MDPI
CY  - Basel
ER  -