TY  - JOUR
A1  - Humber, Michael
A1  - Antoni, Sascha
A1  - Schreiber, Andreas
A1  - Rodes, Berta
A1  - Soriano, Vicente
A1  - Dittmar, Matthias
A1  - Dietrich, Ursula
T1  - Phage display selection of HIV specific conserved mimotopes with IgG from long-term non-progressors
T2  - Retrovirology
N2  - Poster presentation Background The aim of this study is to identify conserved epitopes of HIV-1 neutralizing antibodies in polyclonal plasma from LTNP to finally derive vaccine candidates. Materials and methods The presence of neutralizing antibodies in 9 LTNP sera was proved by in vitro neutralization assays. Phage displayed peptide libraries were screened with LTNP IgG. HIV-specific mimotopes were analyzed for homology to the gp120 structure by a software (3DEX) especially developed for this purpose. Mice were immunized with interesting phages and their sera were analyzed for neutralizing activities against HIV-1. Results After biopannings, between 19% and 75% HIV-specific phage clones were identified by ELISA. Mimotope sequences were identified and could be aligned by 3DEX to linear or conformational epitopes on gp120. A peptide specific immune response was detected in sera of immunized mice. The first mice sera analyzed showed neutralizing activities against HIV-1. Conclusion Mimotopes could be selected from LTNP sera that represent conformational epitopes on gp120. Those ones inducing neutralizing antibodies upon immunization potentially are suited to derive vaccine candidates.
Y1  - 2005
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/1035
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-44519
SN  - 1742-4690
N1  - licensee BioMed Central Ltd.
VL  - 2
IS  - (Suppl 1):P29
SP  - 1
EP  - 1
PB  - BioMed Central
CY  - London
ER  -