TY  - INPR
A1  - Hinzke, Tjorven
A1  - Kleiner, Manuel
A1  - Meister, Mareike
A1  - Schlüter, Rabea
A1  - Hentschker, Christian
A1  - Pané-Farré, Jan
A1  - Hildebrandt, Petra
A1  - Felbeck, Horst
A1  - Sievert, Stefan M.
A1  - Bonn, Florian
A1  - Völker, Uwe
A1  - Becher, Dörte
A1  - Schweder, Thomas
A1  - Markert, Stephanie
T1  - Metabolic differences between symbiont subpopulations in the deep-sea tubeworm Riftia pachyptila
T2  - bioRxiv
N2  - The hydrothermal vent tube worm Riftia pachyptila lives in intimate symbiosis with intracellular sulfur-oxidizing gammaproteobacteria. Although the symbiont population consists of a single 16S rRNA phylotype, bacteria in the same host animal exhibit a remarkable degree of metabolic diversity: They simultaneously utilize two carbon fixation pathways and various energy sources and electron acceptors. Whether these multiple metabolic routes are employed in the same symbiont cells, or rather in distinct symbiont subpopulations, was unclear. As Riftia symbionts vary considerably in cell size and shape, we enriched individual symbiont cell sizes by density gradient centrifugation in order to test whether symbiont cells of different sizes show different metabolic profiles. Metaproteomic analysis and statistical evaluation using clustering and random forests, supported by microscopy and flow cytometry, strongly suggest that Riftia symbiont cells of different sizes represent metabolically dissimilar stages of a physiological differentiation process: Small symbionts actively divide and may establish cellular symbiont-host interaction, as indicated by highest abundance of the cell division key protein FtsZ and highly abundant chaperones and porins in this initial phase. Large symbionts, on the other hand, apparently do not divide, but still replicate DNA, leading to DNA endoreduplication. Highest abundance of enzymes for CO2 fixation, carbon storage and biosynthesis in large symbionts indicates that in this late differentiation stage the symbiont’s metabolism is efficiently geared towards the production of organic material. We propose that this division of labor between smaller and larger symbionts benefits the productivity of the symbiosis as a whole.
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72727
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-727276
UR  - https://www.biorxiv.org/content/10.1101/2020.04.08.032177v1
IS  - 2020.04.08.032177 Version 1
PB  - bioRxiv
ER  -