TY  - JOUR
A1  - Tajnik, Mojca
A1  - Vigilante, Alessandra
A1  - Braun, Simon
A1  - Hänel, Heike
A1  - Luscombe, Nicholas M.
A1  - Ule, Jernej
A1  - Zarnack, Katharina
A1  - König, Julian
T1  - Intergenic Alu exonisation facilitates the evolution of tissue-specific transcript ends
T2  - Nucleic acids research
N2  - The 3′ untranslated regions (3′ UTRs) of transcripts serve as important hubs for posttranscriptional gene expression regulation. Here, we find that the exonisation of intergenic Alu elements introduced new terminal exons and polyadenylation sites during human genome evolution. While Alu exonisation from introns has been described previously, we shed light on a novel mechanism to create alternative 3′ UTRs, thereby opening opportunities for differential posttranscriptional regulation. On the mechanistic level, we show that intergenic Alu exonisation can compete both with alternative splicing and polyadenylation in the upstream gene. Notably, the Alu-derived isoforms are often expressed in a tissue-specific manner, and the Alu-derived 3′ UTRs can alter mRNA stability. In summary, we demonstrate that intergenic elements can affect processing of preceding genes, and elucidate how intergenic Alu exonisation can contribute to tissue-specific posttranscriptional regulation by expanding the repertoire of 3′ UTRs.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/31415
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-314154
UR  - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4666398/
SN  - 1362-4962
SN  - 0305-1048
N1  - (C) The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 43
IS  - 21
SP  - 10492
EP  - 10505
PB  - Oxford
CY  - Oxford Univ. Press
ER  -