TY  - JOUR
A1  - Nagata, Takashi
A1  - Suzuki, Sakura
A1  - Endo, Ryuta
A1  - Shirouzu, Mikako
A1  - Terada, Takaho
A1  - Inoue, Makoto
A1  - Kigawa, Takanori
A1  - Kobayashi, Naohiro
A1  - Güntert, Peter
A1  - Tanaka, Akiko
A1  - Hayashizaki, Yoshihide
A1  - Muto, Yutaka
A1  - Yokoyama, Shigeyuki
T1  - The RRM domain of poly(A)-specific ribonuclease has a noncanonical binding site for mRNA cap analog recognition
T2  - Nucleic acids research
N2  - The degradation of the poly(A) tail is crucial for posttranscriptional gene regulation and for quality control of mRNA. Poly(A)-specific ribonuclease (PARN) is one of the major mammalian 3’ specific exo-ribonucleases involved in the degradation of the mRNA poly(A) tail, and it is also involved in the regulation of translation in early embryonic development. The interaction between PARN and the m7GpppG cap of mRNA plays a key role in stimulating the rate of deadenylation. Here we report the solution structures of the cap-binding domain of mouse PARN with and without the m7GpppG cap analog. The structure of the cap-binding domain adopts the RNA recognition motif (RRM) with a characteristic a-helical extension at its C-terminus, which covers the b-sheet surface (hereafter referred to as PARN RRM). In the complex structure of PARN RRM with the cap analog, the base of the N7-methyl guanosine (m7G) of the cap analog stacks with the solvent-exposed aromatic side chain of the distinctive tryptophan residue 468, located at the C-terminal end of the second b-strand. These unique structural features in PARN RRM reveal a novel cap-binding mode, which is distinct from the nucleotide recognition mode of the canonical RRM domains.
KW  - binding sites
KW  - gene expression regulation
KW  - guanosine
KW  - helix (snails)
KW  - mammals
KW  - multiple sclerosis
KW  - relapsing-remitting
KW  - nucleotides
KW  - quality control
KW  - rna
KW  - messenger
KW  - solvents
KW  - tryptophan
KW  - mice
KW  - ribonucleases
KW  - embryologic development
KW  - titration method
KW  - catabolism
KW  - rna recognition motif
Y1  - 2008
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/5840
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-57809
SN  - 0305-1048
SN  - 1362-4962
N1  - © 2008 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 36
IS  - 14
SP  - 4754
EP  - 4767
PB  - Oxford Univ. Press
CY  - Oxford
ER  -