TY  - JOUR
A1  - Wang, Jinping
A1  - Wang, Ping
A1  - Gui, Shuiqing
A1  - Li, Yun
A1  - Chen, Runhua
A1  - Zeng, Renqing
A1  - Zhao, Peiyan
A1  - Wu, Hanwei
A1  - Huang, Zheyu
A1  - Wu, Jianlong
T1  - Hydroxysafflor yellow a attenuates the apoptosis of peripheral blood CD4+ T lymphocytes in a murine model of sepsis
T2  - Frontiers in pharmacology
N2  - Sepsis is generally considered as a severe condition of inflammation that leads to lymphocyte apoptosis and multiple organ dysfunction. Hydroxysafflor yellow A (HSYA) exerts anti-inflammatory and anti-apoptotic effects in infectious diseases. However, the therapeutic effect of HSYA on polymicrobial sepsis remains unknown. This study was undertaken to investigate the therapeutic effects and the mechanisms of action of HSYA on immunosuppression in a murine model of sepsis induced by cecal ligation and puncture (CLP). NIH mice were randomly divided into four groups: control group, sham group, CLP group, and CLP+HSYA group. HSYA (120 mg/kg) was intravenously injected into experimental mice at 12 h before CLP, concurrent with CLP and 12 h after CLP. The levels of circulating inflammatory cytokines, the apoptosis of CD4+ and CD8+ T lymphocytes, and protein expression of cytochrome C (Cytc), Bax, Bcl-2, cleaved caspase-9, and cleaved caspase-3 were examined. Plasma levels of IL-6, IL-10 and TNF-alpha as well as the apoptosis of CD4+ T lymphocytes were increased compared with sham group. These changes were accompanied by increases of pro-apoptotic proteins including Cytc, Bax, cleaved caspase-9, and cleaved caspase-3 and decreases of anti-apoptotic protein Bcl-2 in CD4+ T lymphocytes from mice undergoing CLP. In contrast, we fail to observe significant effect of HSYA on the apoptosis of CD8+ T lymphocytes in CLP-treated group. Of note, HSYA treatment reversed all above changes observed in CD4+ T lymphocytes, and significantly increased the ratio of CD4+:CD8+ T lymphocytes in CLP-treated mice. In conclusion, HSYA was an effective therapeutic agent in ameliorating sepsis-induced apoptosis of CD4+ T lymphocytes probably through its anti-inflammatory and anti-apoptotic effects.
KW  - hydroxysafflor yellow A
KW  - sepsis
KW  - CD4+ T lymphocytes
KW  - apoptosis
KW  - caspase
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/44537
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-445375
SN  - 1663-9812
N1  - Copyright © 2017 Wang, Wang, Gui, Li, Chen, Zeng, Zhao, Wu, Huang and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
VL  - 8
IS  - Art. 613
SP  - 1
EP  - 10
PB  - Frontiers Media
CY  - Lausanne
ER  -