TY  - JOUR
A1  - Raue, Rebecca Karin
A1  - Frank, Ann-Christin
A1  - Fuhrmann, Dominik Christian
A1  - de la Cruz-Ojeda, Patricia
A1  - Rösser, Silvia
A1  - Bauer, Rebekka
A1  - Cardamone, Giulia
A1  - Weigert, Andreas
A1  - Syed, Shahzad Nawaz
A1  - Schmid, Tobias
A1  - Brüne, Bernhard
T1  - MicroRNA-200c attenuates the tumor-infiltrating capacity of macrophages
T2  - Biology
N2  - Macrophages constitute a major part of the tumor-infiltrating immune cells. Within the tumor microenvironment, they acquire an alternatively activated, tumor-supporting phenotype. Factors released by tumor cells are crucial for the recruitment of tumor-associated macrophages. In the present project, we aimed to understand the role of hsa-miR-200c-3p (miR-200c) in the interplay between tumor cells and macrophages. To this end, we employed a coculture system of MCF7 breast tumor cells and primary human macrophages and observed the transfer of miR-200c from apoptotic tumor cells to macrophages, which required intact CD36 receptor in macrophages. We further comprehensively determined miR-200c targets in macrophages by mRNA-sequencing and identified numerous migration-associated mRNAs to be downregulated by miR-200c. Consequently, miR-200c attenuated macrophage infiltration into 3-dimensional tumor spheroids. miR-200c-mediated reduction in infiltration further correlated with a miR-200c migration signature comprised of the four miR-200c-repressed, predicted targets PPM1F, RAB11FIB2, RDX, and MSN.
KW  - macrophage
KW  - breast tumor
KW  - miR
KW  - tumor microenvironment
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/82506
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-825066
SN  - 2079-7737
N1  - This work was supported by the DFG (GRK 2336 TP06).
N1  - The sequencing data presented in this study are openly available in GEO under accession number GSE195587.
VL  - 11
IS  - 3, art. 349
SP  - 1
EP  - 16
PB  - MDPI
CY  - Basel
ER  -