TY  - JOUR
A1  - Deniz, Erhan
A1  - Löffler, Jan. G.
A1  - Kondratiev, Alexander
A1  - Thun, Asok Rafael
A1  - Shen, Yunxia
A1  - Wille, Georg
A1  - Bredenbeck, Jens
T1  - High-precision background correction and artifact suppression for ultrafast spectroscopy by quasi-simultaneous measurements in a split-sample cell
T2  - Review of scientific instruments
N2  - Alternating acquisition of background and sample spectra is often employed in conventional Fourier-transform infrared spectroscopy or ultraviolet–visible spectroscopy for accurate background subtraction. For example, for solvent background correction, typically a spectrum of a cuvette with solvent is measured and subtracted from a spectrum of a cuvette with solvent and solute. Ultrafast spectroscopies, though, come with many peculiarities that make the collection of well-matched, subtractable background and sample spectra challenging. Here, we present a demountable split-sample cell in combination with a modified Lissajous scanner to overcome these challenges. It allows for quasi-simultaneous measurements of background and sample spectra, mitigating the effects of drifts of the setup and maintaining the beam and sample geometry when swapping between background and sample measurements. The cell is moving between subsequent laser shots to refresh the excited sample volume. With less than 45 μl of solution for 150 μm optical thickness, sample usage is economical. Cell assembly is a key step and covered in an illustrated protocol.
KW  - Sensors
KW  - Image processing
KW  - Fourier transform spectroscopy
KW  - Optical properties
KW  - Thermo optic effects
KW  - Ultrafast laser spectroscopy
KW  - Visible spectroscopy
KW  - Thermal lensing
KW  - Proteins
KW  - Cell assembly
Y1  - 2022
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/75755
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-757553
SN  - 1089-7623
N1  - Funding: DFG ; BR 3746/4-1
N1  - Funding: DFG ; INST 161/722-1
VL  - 93
IS  - 3, art. 033001
SP  - 1
EP  - 6
PB  - American Institute of Physics
CY  - [Erscheinungsort nicht ermittelbar]
ER  -