TY  - JOUR
A1  - Ali, Tamer
A1  - Rogala, Sandra
A1  - Krause, Nina M.
A1  - Bains, Jasleen Kaur
A1  - Melissari, Maria-Theodora
A1  - Währisch, Sandra
A1  - Schwalbe, Harald
A1  - Herrmann, Bernhard
A1  - Grote, Phillip
T1  - Fendrr synergizes with Wnt signalling to regulate fibrosis related genes during lung development via its RNA:dsDNA triplex element
T2  - Nucleic acids research
N2  - Long non-coding RNAs are a very versatile class of molecules that can have important roles in regulating a cells function, including regulating other genes on the transcriptional level. One of these mechanisms is that RNA can directly interact with DNA thereby recruiting additional components such as proteins to these sites via an RNA:dsDNA triplex formation. We genetically deleted the triplex forming sequence (FendrrBox) from the lncRNA Fendrr in mice and found that this FendrrBox is partially required for Fendrr function in vivo. We found that the loss of the triplex forming site in developing lungs causes a dysregulation of gene programs associated with lung fibrosis. A set of these genes contain a triplex site directly at their promoter and are expressed in lung fibroblasts. We biophysically confirmed the formation of an RNA:dsDNA triplex with target promoters in vitro. We found that Fendrr with the Wnt signalling pathway regulates these genes, implicating that Fendrr synergizes with Wnt signalling in lung fibrosis.
Y1  - 2023
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/85687
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-856875
SN  - 0305-1048
VL  - 51
IS  - 12
SP  - 6227
EP  - 6237
PB  - Oxford Univ. Press
CY  - Oxford
ER  -