TY  - JOUR
A1  - Yang, Jiwen
A1  - Wagner, Sebastian Alexander
A1  - Beli, Petra
T1  - Illuminating spatial and temporal organization of protein interaction networks by mass spectrometry-based proteomics
T2  - Frontiers in genetics
N2  - Protein–protein interactions are at the core of all cellular functions and dynamic alterations in protein interactions regulate cellular signaling. In the last decade, mass spectrometry (MS)-based proteomics has delivered unprecedented insights into human protein interaction networks. Affinity purification-MS (AP-MS) has been extensively employed for focused and high-throughput studies of steady state protein–protein interactions. Future challenges remain in mapping transient protein interactions after cellular perturbations as well as in resolving the spatial organization of protein interaction networks. AP-MS can be combined with quantitative proteomics approaches to determine the relative abundance of purified proteins in different conditions, thereby enabling the identification of transient protein interactions. In addition to affinity purification, methods based on protein co-fractionation have been combined with quantitative MS to map transient protein interactions during cellular signaling. More recently, approaches based on proximity tagging that preserve the spatial dimension of protein interaction networks have been introduced. Here, we provide an overview of MS-based methods for analyzing protein–protein interactions with a focus on approaches that aim to dissect the temporal and spatial aspects of protein interaction networks.
KW  - mass spectrometry-based proteomics
KW  - protein–protein interactions
KW  - transient interactions
KW  - spatial interactions
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/51777
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-517778
N1  - Copyright © 2015 Yang, Wagner and Beli. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
VL  - 6
IS  - art. 344
PB  - Frontiers Research Foundation
CY  - Lausanne
ER  -