TY  - JOUR
A1  - Bohnsack, Markus
A1  - Kos, Martin
A1  - Tollervey, David
T1  - Quantitative analysis of snoRNA association with pre-ribosomes and release of snR30 by Rok1 helicase
T2  - EMBO reports
N2  - In yeast, three small nucleolar RNAs (snoRNAs) are essential for the processing of pre-ribosomal RNA—U3, U14 and snR30—whereas 72 non-essential snoRNAs direct site-specific modification of pre-rRNA. We applied a quantitative screen for alterations in the pre-ribosome association to all 75 yeast snoRNAs in strains depleted of eight putative helicases implicated in 40S subunit synthesis. For the modification-guide snoRNAs, we found no clear evidence for the involvement of these helicases in the association or dissociation of pre-ribosomes. However, the DEAD box helicase Rok1 was required specifically for the release of snR30. Point mutations in motif I, but not in motif III, of the helicase domain of Rok1 impaired the release of snR30, but this was less marked than in strains depleted of Rok1, and resulted in a dominant-negative growth phenotype. Dissociation of U3 and U14 from pre-ribosomes is also dependent on helicases, suggesting that release of the essential snoRNAs might differ mechanistically from release of the modification-guide snoRNAs. Keywords: ribosome biogenesis; RNA helicase; snoRNA
KW  - ibosome biogenesis
KW  - RNA helicase
KW  - snoRNA
Y1  - 2008
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/7149
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-62043
N1  - This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation without specific permission. https://creativecommons.org/licenses/by-nc-sa/3.0/
VL  - 9
IS  - 12
SP  - 1260
EP  - 1236
ER  -