TY  - JOUR
A1  - Döhler, Günter
T1  - Aminotransferase-Aktivität in der Blaualge Anacystis nidulans
T1  - Activity of aminotransferases in the blue green alga anacystis nidulans
T2  - Zeitschrift für Naturforschung, C
N2  - The blue-green alga Anacystis nidulans (strain L 1402-1) was grown at + 37 °C in air (0.03 vol.% CO2 and in air enriched with 3.0 vol.% CO2. The effects of several inhibitors on the activity of aminotransferases, 14CO2 fixation and radioactive photosynthetic products of Anacystis were studied. No serine-pyruvate aminotransferase activity could be found in 10-2 м isonicotinyl hydrazide (INH) ; under the influence of this inhibitor aspartate and alanine aminotransferase were decreased about 49% respectively 17.6%. Serine-pyruvate and alanine aminotransferase activity decreased to more than 50% in 10-3 м glyoxalbisulfite. The obtained inhibitory effect of 10-4 м HPMS on serine-piruvate aminotransferase (35%) was stronger than on the other aminotransferases. DCMU (5 × 10-6 м) inhibition on alanine aminotransferase activity was 83.7%. Under the influence of 10-3 м glyoxalbisulfite no 14C-labelled amino acids could be detected after 5 min photosynthesis; 14C-labelling of phosphoenolpyruvate, malate, phosphoglycolate and glycolic acid increased. Isonicotinyl hydrazide (10-2 м) caused in comparison to the control experiment a lower radioactivity in aspartate, glutamate and phosphoenolpyruvate. The results are discussed with reference to the operation of the glycolate pathway and a carboxylation reaction of phosphoenolpyruvate in the blue-green alga Anacystis nidulans.
KW  - Aminotransferase Activity
KW  - Inhibitors
KW  - 14CO2 Fixation
KW  - Anacystis
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72080
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-720809
SN  - 0939-5075
SN  - 1865-7125
VL  - 31.1976
IS  - 7-8
SP  - 433
EP  - 435
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -