TY  - JOUR
A1  - Markert, Claus
A1  - Betz, Brigitte
A1  - Träger, Lothar
T1  - Testosterone metabolism in Streptomyces hydrogenans
T2  - Zeitschrift für Naturforschung, C
N2  - Testosterone, Androst-4-en-3,17-dione, Enzyme Induction, S trep to m yces hydrogenans After cultivation of S trep to m yces hydrogenan s in the presence of 3H-labelled testosterone, radio­ active steroids were extracted separately from the cytosolic, ribosomal and cell wall-membrane fraction of the cells and from the culture medium, respectively.. The separation of the steroids was performed by one-and two-dimensional thin layer chromatography (TLC). The identification of the main metabolites was achieved by crystallization to constant specific radioactivity, specific staining procedures and acetylation. The oxidation of testosterone to androst-4-en-3,17-dione is by far the predominating reaction, which is almost finished after 3 h cultivation. Androst-4-en-3,17-dione is mainly transferred into the culture medium and partly accumulated within the cell wall-membrane fraction. High polar steroid metabolites and androstane derivatives are present in very small amounts only.
KW  - Testosterone
KW  - Androst-4-en-3,17-dione
KW  - Enzyme Induction
KW  - Streptomyces hydrogenans
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/81797
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-817978
SN  - 0939-5075
SN  - 1865-7125
VL  - 30.1975
IS  - 3-4
SP  - 266
EP  - 270
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -