TY  - JOUR
A1  - Sandmann, Gerhard
A1  - Hilgenberg, Wilhelm
T1  - Phosphoenolpyruvatcarboxykinase aus Phycomyces blakesleeanus Bgff.
T1  - Phosphoenolpyruvate carboxykinase in Phycomyces blakesleeanus Bgff.
T2  - Zeitschrift für Naturforschung, C
N2  - Phosphoenolpyruvate carboxykinase (PEPCK) from Phycomyces blakesleeanus was partially purified by protamine sulfate precipitation, ammoniumsulfate precipitation, and diethylamino ethyl cellulose (DEAE) treatment. This preparation was employed for the characterization of the enzyme. The Km values for phosphoenolpyruvate (PEP) and ADP were determined as 1.6 and 0.42 mᴍ. The nucleotid specifity was demonstrated for ADP exclusively. The use of sulfuryl reagents showed the presence of thiol groups sensitive against p-hydroxymercuribenzoate but not effected by N-ethylmaleimide.
KW  - Phycomyces blakesleeanus
KW  - Phosphoenolpyruvate Carboxykinase
KW  - Km values
KW  - Nucleotid Specifity
KW  - Sulfuryl Reagents
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72079
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-720799
SN  - 0939-5075
SN  - 1865-7125
VL  - 33.1978
IS  - 9-10
SP  - 667
EP  - 670
PB  - Verlag der Zeitschrift für Naturforschung
CY  - Tübingen
ER  -