TY  - JOUR
A1  - Mattingly, Matthew
A1  - Weineck, Kristin
A1  - Costa, Jennifer
A1  - Cooper, Robin L.
T1  - Hyperpolarization by activation of halorhodopsin results in enhanced synaptic transmission : Neuromuscular junction and CNS circuit
T2  - PLoS one
N2  - Optogenetics offers a unique method to regulate the activity of select neural circuits. However, the electrophysiological consequences of targeted optogenetic manipulation upon the entire circuit remain poorly understood. Analysis of the sensory-CNS-motor circuit in Drosophila larvae expressing eHpHR and ChR2-XXL revealed unexpected patterns of excitability. Optical stimulation of motor neurons targeted to express eNpHR resulted in inhibition followed by excitation of body wall contraction with repetitive stimulation in intact larvae. In situ preparations with direct electrophysiological measures showed an increased responsiveness to excitatory synaptic activity induced by sensory stimulation within a functional neural circuit. To ensure proper function of eNpHR and ChR2-XXL they were expressed in body wall muscle and direct electrophysiological measurements were obtained. Under eNpHR induced hyperpolarization the muscle remained excitable with increased amplitude of excitatory postsynaptic synaptic potentials. Theoretical models to explain the observations are presented. This study aids in increasing the understanding of the varied possible influences with light activated proteins within intact neural circuits.
KW  - Motor neurons
KW  - Larvae
KW  - Light pulses
KW  - Muscle electrophysiology
KW  - Neural pathways
KW  - Membrane potential
KW  - Nerve fibers
KW  - Muscle fibers
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/46871
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-468714
SN  - 1932-6203
N1  - Copyright: © 2018 Mattingly et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 13
IS  - (7): e0200107
SP  - 1
EP  - 20
PB  - PLoS
CY  - Lawrence, Kan.
ER  -