TY  - INPR
A1  - Narayanasamy, Kaarjel K.
A1  - Rahm, Johanna V.
A1  - Tourani, Siddharth
A1  - Heilemann, Mike
T1  - Fast DNA-PAINT imaging using a deep neural network
T2  - bioRxiv
N2  - DNA points accumulation for imaging in nanoscale topography (DNA-PAINT) is a super-resolution technique with relatively easy-to-implement multi-target imaging. However, image acquisition is slow as sufficient statistical data has to be generated from spatio-temporally isolated single emitters. Here, we trained the neural network (NN) DeepSTORM to predict fluorophore positions from high emitter density DNA-PAINT data. This achieves image acquisition in one minute. We demonstrate multi-color super-resolution imaging of structure-conserved semi-thin neuronal tissue and imaging of large samples. This improvement can be integrated into any single-molecule microscope and enables fast single-molecule super-resolution microscopy.
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/72974
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-729741
IS  - 2021.11.20.469366
ER  -