TY  - JOUR
A1  - Nasiri, Amir Hossein
A1  - Wurm, Jan Philip
A1  - Immer, Carina
A1  - Weickhmann, Anna Katharina
A1  - Wöhnert, Jens
T1  - An intermolecular G-quadruplex as the basis for GTP recognition in the class V–GTP aptamer
T2  - RNA
N2  - Many naturally occurring or artificially created RNAs are capable of binding to guanine or guanine derivatives with high affinity and selectivity. They bind their ligands using very different recognition modes involving a diverse set of hydrogen bonding and stacking interactions. Apparently, the potential structural diversity for guanine, guanosine, and guanine nucleotide binding motifs is far from being fully explored. Szostak and coworkers have derived a large set of different GTP-binding aptamer families differing widely in sequence, secondary structure, and ligand specificity. The so-called class V–GTP aptamer from this set binds GTP with very high affinity and has a complex secondary structure. Here we use solution NMR spectroscopy to demonstrate that the class V aptamer binds GTP through the formation of an intermolecular two-layered G-quadruplex structure that directly incorporates the ligand and folds only upon ligand addition. Ligand binding and G-quadruplex formation depend strongly on the identity of monovalent cations present with a clear preference for potassium ions. GTP binding through direct insertion into an intermolecular G-quadruplex is a previously unobserved structural variation for ligand-binding RNA motifs and rationalizes the previously observed specificity pattern of the class V aptamer for GTP analogs.
KW  - RNA structure
KW  - GTP
KW  - aptamer
KW  - NMR
KW  - G-quadruplex
KW  - ligand binding
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/42512
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-425126
SN  - 1355-8382
SN  - 1469-9001
N1  - © 2016 Nasiri et al. This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a CreativeCommons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
VL  - 22
IS  - 11
SP  - 1750
EP  - 1759
PB  - Laboratory Press ; HighWire Press
CY  - Cold Spring Harbor, NY ; Stanford, Calif.
ER  -