TY  - JOUR
A1  - Leiblein, Maximilian
A1  - Kolb, Tobias
A1  - Christian, Lion Georg
A1  - Schröder, Katrin
A1  - Yaman, Ceyhan
A1  - Schaible, Alexander
A1  - Marzi, Ingo
A1  - Henrich, Dirk
A1  - Janko, Maren Carina
T1  - Introduction of a new surgical method to improve bone healing in a large bone defect by replacement of the induced membrane by a human decellularized dermis repopulated with bone marrow mononuclear cells in rat
T2  - Materials
N2  - The Masquelet technique for the treatment of large bone defects is a two-stage procedure based on an induced membrane. We eliminate the first surgical step by using a decellularized dermal skin graft (Epiflex®) populated with bone marrow mononuclear cells (BMC), as a replacement for the induced membrane. The aim of this study was to demonstrate the feasibility of this technology and provide evidence of equivalent bone healing in comparison to the induced membrane-technique. Therefore, 112 male Sprague–Dawley rats were allocated in six groups and received a 10 mm femoral defect. Defects were treated with either the induced membrane or decellularized dermis, with or without the addition of BMC. Defects were then filled with a scaffold (β-TCP), with or without BMC. After a healing time of eight weeks, femurs were taken for histological, radiological and biomechanical analysis. Defects treated with Epiflex® showed increased mineralization and bone formation predominantly in the transplanted dermis surrounding the defect. No significant decrease of biomechanical properties was found. Vascularization of the defect could be enhanced by addition of BMC. Considering the dramatic reduction of a patient’s burden by the reduced surgical stress and shortened time of treatment, this technique could have a great impact on clinical practice.
KW  - bone healing
KW  - bone marrow mononuclear cells
KW  - human decellularized dermis
KW  - induced membrane
Y1  - 2020
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/54878
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-548781
SN  - 1996-1944
VL  - 13
IS  - 2629
PB  - MDPI
CY  - Basel
ER  -