TY  - JOUR
A1  - Sommer, Julian
A1  - Gerbracht, Kristina M.
A1  - Krause, Felix F.
A1  - Wild, Florian
A1  - Tietgen, Manuela
A1  - Riedel-Christ, Sara
A1  - Sattler, Janko
A1  - Hamprecht, Axel
A1  - Kempf, Volkhard A. J.
A1  - Göttig, Stephan
T1  - OXA-484, an OXA-48-Type Carbapenem-Hydrolyzing Class D β-Lactamase From Escherichia coli
T2  - Frontiers in microbiology
N2  - OXA-48-like carbapenemases are among the most frequent carbapenemases in Gram-negative Enterobacterales worldwide with the highest prevalence in the Middle East, North Africa and Europe. Here, we investigated the so far uncharacterized carbapenemase OXA-484 from a clinical E. coli isolate belonging to the high-risk clone ST410 regarding antibiotic resistance pattern, horizontal gene transfer (HGT) and genetic support. OXA-484 differs by the amino acid substitution 214G compared to the most closely related variants OXA-181 (214R) and OXA-232 (214S). The blaOXA–484 was carried on a self-transmissible 51.5 kb IncX3 plasmid (pOXA-484) showing high sequence similarity with plasmids harboring blaOXA–181. Intraspecies and intergenus HGT of pOXA-484 to different recipients occurred at low frequencies of 1.4 × 10–7 to 2.1 × 10–6. OXA-484 increased MICs of temocillin and carbapenems similar to OXA-232 and OXA-244, but lower compared with OXA-48 and OXA-181. Hence, OXA-484 combines properties of OXA-181-like plasmid support and transferability as well as β-lactamase activity of OXA-232.
KW  - OXA-48
KW  - OXA-484
KW  - IncX
KW  - carbapenemases
KW  - beta-lactamases
KW  - Enterobacterales
KW  - plasmid
Y1  - 2021
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/61252
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-612526
SN  - 1664-302X
VL  - 12
IS  - art. 660094
SP  - 1
EP  - 9
PB  - Frontiers Media
CY  - Lausanne
ER  -