TY  - JOUR
A1  - Winslow, Sofia
A1  - Scholz, Anica
A1  - Rappl, Peter
A1  - Brauß, Thilo Felix
A1  - Mertens, Christina
A1  - Jung, Michaela
A1  - Weigert, Andreas
A1  - Brüne, Bernhard
A1  - Schmid, Tobias
T1  - Macrophages attenuate the transcription of CYP1A1 in breast tumor cells and enhance their proliferation
T2  - PLoS one
N2  - While aberrant cells are routinely recognized and removed by immune cells, tumors eventually escape innate immune responses. Infiltrating immune cells are even corrupted by the tumor to acquire a tumor-supporting phenotype. In line, tumor-associated macrophages are well-characterized to promote tumor progression and high levels of tumor-infiltrating macrophages are a poor prognostic marker in breast cancer. Here, we aimed to further decipher the influence of macrophages on breast tumor cells and determined global gene expression changes in three-dimensional tumor spheroids upon infiltration of macrophages. While various tumor-associated mRNAs were upregulated, expression of the cytochrome P450 family member CYP1A1 was markedly attenuated. Repression of CYP1A1 in tumor cells was elicited by a macrophage-shaped tumor microenvironment rather than by direct tumor cell-macrophage contacts. In line with changes in RNA expression profiles, macrophages enhanced proliferation of the tumor cells. Enhanced proliferation and macrophage presence further correlated with reduced CYP1A1 expression in patient tumors when compared with normal tissue. These findings are of interest in the context of combinatory therapeutic approaches involving cytotoxic and immune-modulatory compounds.
KW  - Breast tumors
KW  - Macrophages
KW  - Messenger RNA
KW  - Immune cells
KW  - Breast cancer
KW  - Gene expression
KW  - RNA extraction
KW  - RNA isolation
Y1  - 2019
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/48580
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-485801
SN  - 1932-6203
N1  - Copyright: © 2019 Winslow et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 14
IS  - (1): e0209694
SP  - 1
EP  - 12
PB  - PLoS
CY  - Lawrence, Kan.
ER  -