TY  - JOUR
A1  - Grey, Martin
A1  - Düsterhöft, Andreas
A1  - Henriques, Joao A. P.
A1  - Brendel, Martin
T1  - Allelism of PSO4 and PRP19 links pre-mRNA processing with recombination and error-prone DNA repair in Saccharomyces cerevisia
T2  - Nucleic acids research
N2  - The radiation-sensitive mutant pso4-1 of Saccharomyces cerevisiae shows a pleiotropic phenotype, including sensitivity to DNA cross-linking agents, nearly blocked sporulation and reduced mutability. We have cloned the putative yeast DNA repair gene PSO4 from a genomic library by complementation of the blocked UV-induced mutagenesis and of sporulation in diploids homozygous for pso4-1. Sequence analysis revealed that gene PSO4 consists of 1512 bp located upstream of UBI4 on chromosome XII and encodes a putative protein of 56.7 kDa. PSO4 is allelic to PRP19, a gene encoding a spliceosome-associated protein, but shares no significant homology with other yeast genes. Gene disruption with a destroyed reading frame of our PSO4 clone resulted in death of haploid cells, confirming the finding that PSO4/PRP19 is an essential gene. Thus, PSO4 is the third essential DNA repair gene found in the yeast S.cerevisiae.
Y1  - 1996
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/2143
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-32711
SN  - 1362-4962
SN  - 0305-1048
N1  - Copyright © 1996 by Oxford University Press, http://nar.oxfordjournals.org/misc/terms.dtl
VL  - 24
IS  - 20
SP  - 4009
EP  - 4014
PB  - Oxford Univ. Press
CY  - Oxford
ER  -