TY  - JOUR
A1  - Figge, Marc Thilo
A1  - Garin, Alexandre
A1  - Gunzer, Matthias
A1  - Kosco-Vilbois, Marie
A1  - Toellner, Kai-Michael
A1  - Meyer-Hermann, Michael
T1  - Deriving a germinal center lymphocyte migration model from two-photon data
T2  - Journal of experimental medicine
N2  - Recently, two-photon imaging has allowed intravital tracking of lymphocyte migration and cellular interactions during germinal center (GC) reactions. The implications of two-photon measurements obtained by several investigators are currently the subject of controversy. With the help of two mathematical approaches, we reanalyze these data. It is shown that the measured lymphocyte migration frequency between the dark and the light zone is quantitatively explained by persistent random walk of lymphocytes. The cell motility data imply a fast intermixture of cells within the whole GC in approximately 3 h, and this does not allow for maintenance of dark and light zones. The model predicts that chemotaxis is active in GCs to maintain GC zoning and demonstrates that chemotaxis is consistent with two-photon lymphocyte motility data. However, the model also predicts that the chemokine sensitivity is quickly down-regulated. On the basis of these fi ndings, we formulate a novel GC lymphocyte migration model and propose its verifi cation by new two-photon experiments that combine the measurement of B cell migration with that of specifi c chemokine receptor expression levels. In addition, we discuss some statistical limitations for the interpretation of two-photon cell motility measurements in general.
Y1  - 2008
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/6909
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-69821
SN  - 1540-9358
SN  - 0022-1007
SN  - 1540-9538
N1  - © 2008 Figge et al. This article is distributed under the terms of an Attribution– Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial– Share Alike 3.0 Unported license, as described at http://creativecommons .org/licenses/by-nc-sa/3.0/).
VL  - 205
IS  - 13
SP  - 3019
EP  - 3029
PB  - Rockefeller Univ. Press
CY  - New York, NY
ER  -