TY  - JOUR
A1  - Hänsel, Robert
A1  - Löhr, Frank
A1  - Foldynová-Trantírková, Silvie
A1  - Bamberg, Ernst
A1  - Trantírek, Lukáš
A1  - Dötsch, Volker
T1  - The parallel G-quadruplex structure of vertebrate telomeric repeat sequences is not the preferred folding topology under physiological conditions
T2  - Nucleic acids research
N2  - G-quadruplex topologies of telomeric repeat sequences from vertebrates were investigated in the presence of molecular crowding (MC) mimetics, namely polyethylene glycol 200 (PEG), Ficoll 70 as well as Xenopus laevis egg extract by CD and NMR spectroscopy and native PAGE. Here, we show that the conformational behavior of the telomeric repeats in X. laevis egg extract or in Ficoll is notably different from that observed in the presence of PEG. While the behavior of the telomeric repeat in X. laevis egg extract or in Ficoll resembles results obtained under dilute conditions, PEG promotes the formation of high-order parallel topologies. Our data suggest that PEG should not be used as a MC mimetic.
Y1  - 2011
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/16551
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30-97710
SN  - 1362-4962
SN  - 0305-1048
N1  - © The Author(s) 2011. Published by Oxford University Press. ; This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 39
IS  - 13
SP  - 5768
EP  - 5775
PB  - Oxford Univ. Press
CY  - Oxford
ER  -