TY  - JOUR
A1  - Nickel, Thomas
A1  - Pfeiler, Susanne
A1  - Summo, Claudia
A1  - Kopp, Reinhard
A1  - Meimarakis, Georgios
A1  - Sicic, Zeljka
A1  - Lambert, Marius
A1  - Lackermair, Korbinian Johannes
A1  - David, Robert
A1  - Beiras Fernández, Andrés
A1  - Kaczmarek, Ingo
A1  - Weis, Michael
T1  - oxLDL downregulates the dendritic cell homing factors CCR7 and CCL21
T2  - Mediators of inflammation
N2  - Dendritic cells (DCs) and oxLDL play an important role in the atherosclerotic process with DCs accumulating in the plaques during plaque progression. Our aim was to investigate the role of oxLDL in the modulation of the DC homing-receptor CCR7 and endothelial-ligand CCL21. Methods and Results. The expression of the DC homing-receptor CCR7 and its endothelial-ligand CCL21 was examined on atherosclerotic carotic plaques of 47 patients via qRT-PCR and immunofluorescence. In vitro, we studied the expression of CCR7 on DCs and CCL21 on human microvascular endothelial cells (HMECs) in response to oxLDL. CCL21- and CCR7-mRNA levels were significantly downregulated in atherosclerotic plaques versus non-atherosclerotic controls [90% for CCL21 and 81% for CCR7 (P<0.01)]. In vitro, oxLDL reduced CCR7 mRNA levels on DCs by 30% and protein levels by 46%. Furthermore, mRNA expression of CCL21 was significantly reduced by 50% (P<0.05) and protein expression by 24% in HMECs by oxLDL (P<0.05). Conclusions. The accumulation of DCs in atherosclerotic plaques appears to be related to a downregulation of chemokines and their ligands, which are known to regulate DC migration. oxLDL induces an in vitro downregulation of CCR7 and CCL21, which may play a role in the reduction of DC migration from the plaques.
Y1  - 2012
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/24571
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-245713
SN  - 1466-1861
SN  - 0962-9351
VL  - 2012
IS  - Article ID 320953
PB  - Hindawi Publishing Corp.
CY  - Sylvania, Ohio
ER  -