TY  - JOUR
A1  - Geiger, Florian
A1  - Beverungen, Mirjam
A1  - Lorenz, Helga
A1  - Wieland, Julia
A1  - Fehr, Michael
A1  - Kasten, Philip
T1  - Bone substitute effect on vascularization and bone remodeling after application of phVEGF165 transfected BMSC
T2  - Journal of functional biomaterials
N2  - VEGF (vascular endothelial growth factor) promotes vascularization and remodeling of bone substitutes. The aim of this study was to examine the effect of distinct resorbable ceramic carriers on bone forming capacities of VEGF transfected bone marrow stromal cells (BMSC). A critical size defect of the radius in rabbits was filled either by a low surface scaffold called beta-TCP (tricalciumphsphate) or the high surface scaffold CDHA (calcium deficient hydroxy-apatite) loaded with autologous BMSC, which were either transfected with a control plasmid or a plasmid coding for phVEGF165. They were compared to unloaded scaffolds. Thus, six treatment groups (n = 6 in each group) were followed by X-ray over 16 weeks. After probe retrieval, the volume of new bone was measured by micro-CT scans and vascularization was assessed in histology. While only minor bone formation was found in both carriers when implanted alone, BMSC led to increased osteogenesis in both carriers. VEGF promoted vascularization of the scaffolds significantly in contrast to BMSC alone. Bone formation was increased in the beta-TCP group, whereas it was inhibited in the CDHA group that showed faster scaffold degradation. The results indicate that the interaction of VEGF transfected BMSC with resorbable ceramic carrier influences the ability to promote bone healing.
KW  - angiogenesis
KW  - bone substitutes
KW  - VEGF
KW  - osteogenesis
KW  - BMSC
Y1  - 2012
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/25210
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-252101
SN  - 2079-4983
N1  - © 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
VL  - 3
IS  - 2
SP  - 313
EP  - 326
PB  - Molecular Diversity Preservation International (MDPI)
CY  - Basel
ER  -