TY  - JOUR
A1  - Ventayol, Marina
A1  - Viñas, Jose Luis
A1  - Sola, Anna
A1  - Jung, Michaela
A1  - Brüne, Bernhard
A1  - Pi, Felip
A1  - Mastora, Chrysoula
A1  - Hotter, Georgina
T1  - miRNA let-7e targeting MMP9 is involved in adipose-derived stem cell differentiation toward epithelia
T2  - Cell death & disease
N2  - miRNA let-7e is involved in stem cell differentiation, and metalloproteinases are among its potential target genes. We hypothesized that the inhibitory action of let-7e on regulation of MMP9 expression could represent a crucial mechanism during differentiation of adipose-derived stem cells (ASCs). ASCs were differentiated with all-trans retinoic acid (ATRA) to promote differentiation, and the effect of let-7 silencing during differentiation was tested. Results indicate that ASCs cultured with ATRA differentiated into cells of the epithelial lineage. We found that ASCs cultured with ATRA or transfected with miRNA let-7e expressed epithelial markers such as cytokeratin-18 and early renal organogenesis markers such as Pax2, Wt1, Wnt4 and megalin. Conversely, the specific knockdown of miRNA let-7e in ASCs significantly decreased the expression of these genes, indicating its vital role during the differentiation process. Using luciferase reporter assays, we also showed that MMP9 is a direct target of miRNA let-7e. Thus, our results suggest that miRNA let-7e acts as a matrix metalloproteinase-9 (MMP9) inhibitor and differentiation inducer in ASCs.
KW  - adipose-derived stem cells (ASCs)
KW  - miRNA let-7e
KW  - MMP9
KW  - differentiation
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/32986
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-329864
SN  - 2041-4889
N1  - Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
VL  - 5
IS  - e1048
SP  - 1
EP  - 10
PB  - Nature Publishing Group
CY  - London [u. a.]
ER  -