TY  - JOUR
A1  - Willershausen, Ines
A1  - Barbeck, Mike
A1  - Boehm, Nicole
A1  - Sader, Robert Alexander
A1  - Willershausen, Brita
A1  - Kirkpatrick, Charles James
A1  - Ghanaati, Shahram Michael
T1  - Non-cross-linked collagen type I/III materials enhance cell proliferation : in vitro and in vivo evidence
T2  - Journal of applied oral science
N2  - Objective: To analyze Mucograft (MG), a recently introduced collagen matrix, in vitro and in vivo, and compare it with BioGide (BG), a well-established collagen membrane, as control.
Material and methods: A detailed analysis of the materials surface and ultra-structure was performed. Cellular growth patterns and proliferation rates of human fibroblasts on MG and BG were analyzed in vitro. In addition, the early tissue reaction of CD-1 mouse to these materials was analyzed by means of histological and histomorphometrical analysis.
Results: MG showed a three-fold higher thickness both in dry and wet conditions, when compared to BG. The spongy surface of BG significantly differed from that of MG. Cells showed a characteristic proliferation pattern on the different materials in vitro. Fibroblasts tended to proliferate on the compact layers of both collagens, with the highest values on the compact side of BG. In vivo, at day three both materials demonstrated good tissue integration, with a mononuclear cell sheet of fibroblasts on all surfaces, however, without penetrating into the materials.
Conclusions: The findings of this study showed that MG and BG facilitate cell proliferation on both of their surfaces in vitro. In vivo, these two materials induce a comparable early tissue reaction, while serving as cell occlusive barriers.
KW  - Collagen type I
KW  - Collagen type III
KW  - Guided tissue regeneration
KW  - Bone regeneration
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/33375
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-333756
SN  - 1678-7765
N1  - This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 22
IS  - 1
SP  - 29
EP  - 37
PB  - University of São Paulo, Faculdade de Odontologia de Bauru
CY  - Bauru, São Paulo
ER  -