TY  - JOUR
A1  - Koss, Michael Janusz
A1  - Hoffmann, Janosch
A1  - Nguyen, Nauke
A1  - Pfister, Marcel
A1  - Mischak, Harald
A1  - Mullen, William
A1  - Husi, Holger
A1  - Rejdak, Robert
A1  - Koch, Frank H.
A1  - Jankowski, Joachim
A1  - Krueger, Katharina
A1  - Bertelmann, Thomas
A1  - Klein, Julie
A1  - Schanstra, Joost P.
A1  - Siwy, Justyna
T1  - Proteomics of vitreous humor of patients with exudative age-related macular degeneration
T2  - PLoS One
N2  - Background: There is absence of specific biomarkers and an incomplete understanding of the pathophysiology of exudative age-related macular degeneration (AMD).
Methods and findings: Eighty-eight vitreous samples (73 from patients with treatment naïve AMD and 15 control samples from patients with idiopathic floaters) were analyzed with capillary electrophoresis coupled to mass spectrometry in this retrospective case series to define potential candidate protein markers of AMD. Nineteen proteins were found to be upregulated in vitreous of AMD patients. Most of the proteins were plasma derived and involved in biological (ion) transport, acute phase inflammatory reaction, and blood coagulation. A number of proteins have not been previously associated to AMD including alpha-1-antitrypsin, fibrinogen alpha chain and prostaglandin H2-D isomerase. Alpha-1-antitrypsin was validated in vitreous of an independent set of AMD patients using Western blot analysis. Further systems biology analysis of the data indicated that the observed proteomic changes may reflect upregulation of immune response and complement activity.
Conclusions: Proteome analysis of vitreous samples from patients with AMD, which underwent an intravitreal combination therapy including a core vitrectomy, steroids and bevacizumab, revealed apparent AMD-specific proteomic changes. The identified AMD-associated proteins provide some insight into the pathophysiological changes associated with AMD.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/33796
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-337962
SN  - 1932-6203
N1  - Copyright: © 2014 Koss et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 9
IS  - (5):e96895
PB  - PLoS
CY  - Lawrence, Kan.
ER  -