TY  - JOUR
A1  - Gauvrit, Sébastien
A1  - Philippe, Josette
A1  - Lesage, Matthieu
A1  - Tjwa, Marc
A1  - Godin, Isabelle
A1  - Germain, Stéphane
T1  - The role of RNA interference in the developmental separation of blood and lymphatic vasculature
T2  - Vascular cell
N2  - Background: Dicer is an RNase III enzyme that cleaves double stranded RNA and generates functional interfering RNAs that act as important regulators of gene and protein expression. Dicer plays an essential role during mouse development because the deletion of the dicer gene leads to embryonic death. In addition, dicer-dependent interfering RNAs regulate postnatal angiogenesis. However, the role of dicer is not yet fully elucidated during vascular development.
Methods: In order to explore the functional roles of the RNA interference in vascular biology, we developed a new constitutive Cre/loxP-mediated inactivation of dicer in tie2 expressing cells.
Results: We show that cell-specific inactivation of dicer in Tie2 expressing cells does not perturb early blood vessel development and patterning. Tie2-Cre; dicerfl/fl mutant embryos do not show any blood vascular defects until embryonic day (E)12.5, a time at which hemorrhages and edema appear. Then, midgestational lethality occurs at E14.5 in mutant embryos. The developing lymphatic vessels of dicer-mutant embryos are filled with circulating red blood cells, revealing an impaired separation of blood and lymphatic vasculature.
Conclusion: Thus, these results show that RNA interference perturbs neither vasculogenesis and developmental angiogenesis, nor lymphatic specification from venous endothelial cells but actually provides evidence for an epigenetic control of separation of blood and lymphatic vasculature.
KW  - Lymphangiogenesis
KW  - Veino-lymphatic separation
KW  - Angiogenesis
KW  - RNA interference
KW  - Dicer
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/34384
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-343842
SN  - 2045-824X
N1  - © 2014 Gauvrit et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
VL  - 6
IS  - 9
SP  - 1
EP  - 10
PB  - BioMed Central
CY  - London
ER  -