TY  - JOUR
A1  - Meraviglia, Viviana
A1  - Azzimato, Valerio
A1  - Piacentini, Luca
A1  - Chiesa, Mattia
A1  - Kesharwani, Rupesh K.
A1  - Frati, Caterina
A1  - Capogrossi, Maurizio C.
A1  - Gaetano, Carlo
A1  - Pompilio, Giulio
A1  - Colombo, Gualtiero I.
A1  - Rossini, Alessandra
T1  - Syngeneic cardiac and bone marrow stromal cells display tissue-specific microRNA signatures and microRNA subsets restricted to diverse differentiation processes
T2  - PLoS One
N2  - MicroRNAs are key modulators at molecular level in different biological processes, including determination of cell fate and differentiation. Herein, microRNA expression profiling experiments were performed on syngeneic cardiac (CStC) and bone marrow (BMStC) mesenchymal stromal cells cultured in standard growth medium and then in vitro exposed to adipogenic, osteogenic, cardiomyogenic and endothelial differentiation media. Analysis identified a tissue-specific microRNA signature composed of 16 microRNAs that univocally discriminated cell type of origin and that were completely unaffected by in vitro differentiation media: 4 microRNAs were over-expressed in cardiac stromal cells, and 12 were overexpressed or present only in bone marrow stromal cells. Further, results revealed microRNA subsets specifically modulated by each differentiation medium, irrespective of the cell type of origin, and a subset of 7 microRNAs that were down-regulated by all media with respect to growth medium. Finally, we identified 16 microRNAs that were differentially modulated by the media when comparing the two tissues of origin. The existence of a tissue-specific microRNA signature surviving to any differentiation stimuli, strongly support the role if microRNAs determining cell identity related to tissue origin. Moreover, we identified microRNA subsets modulated by different culture conditions in a tissue-specific manner, pointing out their importance during differentiation processes.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/35103
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-351038
SN  - 1932-6203
N1  - Copyright: © 2014 Meraviglia et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 9
IS  - (9):e107269
PB  - PLoS
CY  - Lawrence, Kan.
ER  -