TY  - JOUR
A1  - Silva, Roberta Lane de Oliveira
A1  - Silva, Manassés Daniel
A1  - Ferreira Neto, José Ribamar Costa
A1  - Nardi, Claudia Huerta de
A1  - Chabregas, Sabrina Moutinho
A1  - Burnquist, William Lee
A1  - Kahl, Günter
A1  - Benko-Iseppon, Ana Maria
A1  - Kido, Ederson Akio
T1  - Validation of novel reference genes for reverse transcription quantitative real-time PCR in drought-stressed sugarcane
T2  - The scientific world journal
N2  - One of the most challenging aspects of RT-qPCR data analysis is the identification of reliable reference genes. Ideally, they should be neither induced nor repressed under different experimental conditions. To date, few reference genes have been adequately studied for sugarcane (Saccharum spp.) using statistical approaches. In this work, six candidate genes (αTUB, GAPDH, H1, SAMDC, UBQ, and 25S rRNA) were tested for gene expression normalization of sugarcane root tissues from drought-tolerant and -sensitive accessions after continuous dehydration (24 h). By undergoing different approaches (GeNorm, NormFinder, and BestKeeper), it was shown that most of them could be used in combinations for normalization purposes, with the exception of SAMDC. Nevertheless three of them (H1, αTUB, and GAPDH) were considered the most reliable reference genes. Their suitability as reference genes validated the expression profiles of two targets (AS and PFPα1), related to SuperSAGE unitags, in agreement with results revealed by previous in silico analysis. The other two sugarcane unitags (ACC oxidase and PIP1-1), after salt stress (100 mM NaCl), presented their expressions validated in the same way. In conclusion, these reference genes will be useful for dissecting gene expression in sugarcane roots under abiotic stress, especially in transcriptomic studies using SuperSAGE or RNAseq approaches.
Y1  - 2014
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/37179
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-371790
SN  - 1537-744X
N1  - Copyright © 2014 Roberta Lane de Oliveira Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2014
IS  - Article ID 357052
SP  - 1
EP  - 12
PB  - Hindawi Publishing Corp.
ER  -