TY  - JOUR
A1  - Albarracín, Virginia Helena
A1  - Kraiselburd, Ivana
A1  - Bamann, Christian
A1  - Wood, Phillip G.
A1  - Bamberg, Ernst
A1  - Farias, Maria E.
A1  - Gärtner, Wolfgang
T1  - Functional green-tuned proteorhodopsin from modern stromatolites
T2  - PLoS one
N2  - The sequenced genome of the poly-extremophile Exiguobacterium sp. S17, isolated from modern stromatolites at Laguna Socompa (3,570 m), a High-Altitude Andean Lake (HAAL) in Argentinean Puna revealed a putative proteorhodopsin-encoding gene. The HAAL area is exposed to the highest UV irradiation on Earth, making the microbial community living in the stromatolites test cases for survival strategies under extreme conditions. The heterologous expressed protein E17R from Exiguobacterium (248 amino acids, 85% sequence identity to its ortholog ESR from E. sibiricum) was assembled with retinal displaying an absorbance maximum at 524 nm, which makes it a member of the green-absorbing PR-subfamily. Titration down to low pH values (eventually causing partial protein denaturation) indicated a pK value between two and three. Global fitting of data from laser flash-induced absorption changes gave evidence for an early red-shifted intermediate (its formation being below the experimental resolution) that decayed (τ1 = 3.5 μs) into another red-shifted intermediate. This species decayed in a two-step process (τ2 = 84 μs, τ3 = 11 ms), to which the initial state of E17-PR was reformed with a kinetics of 2 ms. Proton transport capability of the HAAL protein was determined by BLM measurements. Additional blue light irradiation reduced the proton current, clearly identifying a blue light absorbing, M-like intermediate. The apparent absence of this intermediate is explained by closely matching formation and decay kinetics.
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/41510
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-415105
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4871484/
SN  - 1932-6203
N1  - Copyright: © 2016 Albarracín et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 11
IS  - (5): e0154962
SP  - 1
EP  - 18
PB  - PLoS
CY  - Lawrence, Kan.
ER  -