TY  - JOUR
A1  - D'Imprima, Edoardo
A1  - Mills, Deryck J.
A1  - Parey, Kristian Kurt
A1  - Brandt, Ulrich
A1  - Kühlbrandt, Werner
A1  - Zickermann, Volker
A1  - Vonck, Janet
T1  - Cryo-EM structure of respiratory complex I reveals a link to mitochondrial sulfur metabolism
T2  - Biochimica et Biophysica Acta
N2  - Mitochondrial complex I is a 1MDa membrane protein complex with a central role in aerobic energy metabolism. The bioenergetic core functions are executed by 14 central subunits that are conserved from bacteria to man. Despite recent progress in structure determination, our understanding of the function of the ~30 accessory subunits associated with the mitochondrial complex is still limited. We have investigated the structure of complex I from the aerobic yeast Yarrowia lipolytica by cryo-electron microscopy. Our density map at 7.9Å resolution closely matches the 3.6-3.9Å X-ray structure of the Yarrowia lipolytica complex. However, the cryo-EM map indicated an additional subunit on the side of the matrix arm above the membrane surface, pointing away from the membrane arm. The density, which is not present in any previously described complex I structure and occurs in about 20 % of the particles, was identified as the accessory sulfur transferase subunit ST1. The Yarrowia lipolytica complex I preparation is active in generating H2S from the cysteine derivative 3-mercaptopyruvate, catalyzed by ST1. We thus provide evidence for a link between respiratory complex I and mitochondrial sulfur metabolism.
KW  - Complex I
KW  - Cryo-electron microscopy
KW  - Electron transport chain
KW  - Mitochondria
KW  - NADH:ubiquinone oxidoreductase
KW  - Sulfur metabolism
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/41813
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-418133
SN  - 0005-2728
N1  - © 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
VL  - 1857
IS  - 12
SP  - 1935
EP  - 1942
ER  -