TY  - JOUR
A1  - Grammatikos, Georgios
A1  - Dietz, Julia
A1  - Ferreirós Bouzas, Nerea
A1  - Koch, Alexander
A1  - Dultz, Georg
A1  - Bon, Dimitra
A1  - Karakasiliotis, Ioannis
A1  - Lutz, Thomas
A1  - Knecht, Gaby
A1  - Gute, Peter
A1  - Herrmann, Eva
A1  - Zeuzem, Stefan
A1  - Mavromara, Penelope
A1  - Sarrazin, Christoph
A1  - Pfeilschifter, Josef
T1  - Persistence of HCV in acutely-infected patients depletes C24-ceramide and upregulates sphingosine and sphinganine serum levels
T2  - International journal of molecular sciences
N2  - Hepatitis C virus (HCV) substantially affects lipid metabolism, and remodeling of sphingolipids appears to be essential for HCV persistence in vitro. The aim of the current study is the evaluation of serum sphingolipid variations during acute HCV infection. We enrolled prospectively 60 consecutive patients with acute HCV infection, most of them already infected with human immunodeficiency virus (HIV), and serum was collected at the time of diagnosis and longitudinally over a six-month period until initiation of antiviral therapy or confirmed spontaneous clearance. Quantification of serum sphingolipids was performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Spontaneous clearance was observed in 11 out of 60 patients (18.3%), a sustained viral response (SVR) in 43 out of 45 patients (95.5%) receiving an antiviral treatment after follow-up, whereas persistence of HCV occurred in six out of 60 patients (10%). C24-ceramide (C24-Cer)-levels increased at follow-up in patients with spontaneous HCV eradication (p < 0.01), as compared to baseline. Sphingosine and sphinganine values were significantly upregulated in patients unable to clear HCV over time compared to patients with spontaneous clearance of HCV infection on follow-up (p = 0.013 and 0.006, respectively). In summary, the persistence of HCV after acute infection induces a downregulation of C24Cer and a simultaneous elevation of serum sphingosine and sphinganine concentrations.
KW  - HIV
KW  - hepatitis C
KW  - sphingolipid
KW  - biomarker
KW  - angiopoietin-like 3 (ANGPTL3)
Y1  - 2016
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/42270
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-422706
UR  - https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4926455
SN  - 1661-6596
N1  - © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
VL  - 17
IS  - 6, 922
SP  - 1
EP  - 12
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -