TY  - JOUR
A1  - Brandenstein, Melanie von
A1  - Pütz, Katharina Christine
A1  - Schlosser, Barbara Monika
A1  - Löser, Heike
A1  - Kallinowski, Joachim Peter
A1  - Gödde, Daniel
A1  - Büttner, Reinhard
A1  - Störkel, Stefan
A1  - Fries, Jochen W. U.
T1  - Vimentin 3, the new hope, differentiating RCC versus oncocytoma
T2  - Disease markers
N2  - Vimentin is currently used to differentiate between malignant renal carcinomas and benign oncocytomas. Recent reports showing Vimentin positive oncocytomas seriously question the validity of this present diagnostic approach. Vimentin 3 is a spliced variant and ends with a unique C-terminal ending after exon 7 which differentiates it from the full length version that has 9 exons. Therefore, the protein size is different; the full length Vimentin version has a protein size of ~57 kDa and the truncated version of ~47 kDa. We designed an antibody, called Vim3, against the unique C-terminal ending of the Vimentin 3 variant. Using immune histology, immune fluorescence, Western blot, and qRT-PCR analysis, a Vim3 overexpression was detectable exclusively in oncocytoma, making the detection of Vim3 a potential specific marker for benign kidney tumors. This antibody is the first to clearly differentiate benign oncocytoma and the mimicking eosinophilic variants of the RCCs. This differentiation between malignant and benign RCCs is essential for operative planning, follow-up therapy, and patients' survival. In the future the usage of Vimentin antibodies in routine pathology has to be applied with care. Consideration must be given to Vimentin specific binding epitopes otherwise a misdiagnosis of the patients' tumor samples may result.
Y1  - 2015
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/42382
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-423822
SN  - 1875-8630
N1  - Copyright © 2015 Melanie von Brandenstein  et al. This is an open access article distributed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/ , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
VL  - 2015
IS  - Article ID 368534
PB  - Hindawi
CY  - New York, NY [u.a.]
ER  -