TY  - JOUR
A1  - Singhal, Ravi K.
A1  - Kruse, Christine
A1  - Heidler, Juliana
A1  - Strecker, Valentina
A1  - Zwicker, Klaus
A1  - Düsterwald, Lea
A1  - Westermann, Benedikt
A1  - Herrmann, Johannes
A1  - Wittig, Ilka
A1  - Rapaport, Doron
T1  - Coi1 is a novel assembly factor of the yeast complex III–complex IV supercomplex
T2  - Molecular biology of the cell
N2  - The yeast bc1 complex (complex III) and cytochrome oxidase (complex IV) are mosaics of core subunits encoded by the mitochondrial genome and additional nuclear-encoded proteins imported from the cytosol. Both complexes build in the mitochondrial inner membrane various supramolecular assemblies. The formation of the individual complexes and their supercomplexes depends on the activity of dedicated assembly factors. We identified a so far uncharacterized mitochondrial protein (open reading frame YDR381C-A) as an important assembly factor for complex III, complex IV, and their supercomplexes. Therefore, we named this protein Cox interacting (Coi) 1. Deletion of COI1 results in decreased respiratory growth, reduced membrane potential, and hampered respiration, as well as slow fermentative growth at low temperature. In addition, coi1Δ cells harbour reduced steady-state levels of subunits of complexes III and IV as well as of the assembled complexes and supercomplexes. Interaction of Coi1 with respiratory chain subunits seems transient, as it appears to be a stoichiometric subunit neither of complex III nor of complex IV. Collectively, this work identifies a novel protein that plays a role in the assembly of the mitochondrial respiratory chain.
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45062
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-450621
SN  - 1939-4586
SN  - 1059-1524
N1  - October 1, 2017 © 2017 Singhal et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).
VL  - 28
IS  - 20
SP  - 2609
EP  - 2622
PB  - American Society for Cell Biology
CY  - Bethesda, Md.
ER  -