TY  - JOUR
A1  - Kakoschky, Bianca
A1  - Pleli, Thomas
A1  - Schmithals, Christian
A1  - Zeuzem, Stefan
A1  - Brüne, Bernhard
A1  - Vogl, Thomas J.
A1  - Korf, Horst-Werner
A1  - Weigert, Andreas
A1  - Piiper, Albrecht
T1  - Selective targeting of tumor associated macrophages in different tumor models
T2  - PLoS one
N2  - Tumor progression largely depends on the presence of alternatively polarized (M2) tumor-associated macrophages (TAMs), whereas the classical M1-polarized macrophages can promote anti-tumorigenic immune responses. Thus, selective inhibition of M2-TAMs is a desirable anti-cancer approach in highly resistant tumor entities such as hepatocellular carcinoma (HCC) or breast cancer. We here examined whether a peptide that selectively binds to and is internalized by in vitro-differentiated murine M2 macrophages as compared to M1 macrophages, termed M2pep, could be used to selectively target TAMs in HCC and breast carcinoma. We confirmed selectivity of M2pep for in vitro M2 polarized macrophages. Upon incubation of suspended mixed 4T1 tumor cells with M2pep, high amounts of the TAMs were found to be associated with M2pep, whereas in mixed tumor cell suspensions from two HCC mouse models, M2pep showed only low-degree binding to TAMs. M2pep also showed low-degree targeting of liver macrophages. This indicates that the TAMs in different tumor entities show different targeting of M2pep and that M2pep is a very promising approach to develop selective M2-TAM-targeting in tumor entities containing M2-TAMs with significant amounts of the so far elusive M2pep receptor(s).
KW  - Macrophages
KW  - Mouse models
KW  - Cell binding
KW  - Flow cytometry
KW  - Breast tumors
KW  - Hepatocellular carcinoma
KW  - Cell staining
KW  - Kupffer cells
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/45736
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-457363
SN  - 1932-6203
N1  - Copyright: © 2018 Kakoschky et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
VL  - 13
IS  - (2): e0193015
SP  - 1
EP  - 13
PB  - PLoS
CY  - Lawrence, Kan.
ER  -