TY  - JOUR
A1  - Hötte, Katharina
A1  - Smyrek, Isabell
A1  - Starzinski-Powitz, Anna
A1  - Stelzer, Ernst H. K.
T1  - Endogenous AJAP1 associates with the cytoskeleton and attenuates angiogenesis in endothelial cells
T2  - Biology open
N2  - The adherens junction associated protein 1 (AJAP1, aka shrew-1) is presumably a type-I transmembrane protein localizing and interacting with the E-cadherin-catenin complex. In various tumors, AJAP1 expression is reduced or lost, including hepatocellular and esophageal squamous cell carcinoma, and glial-derived tumors. The aberrant expression of AJAP1 is associated with alterations in cell migration, invasion, increased tumor growth, and tumor vascularization, suggesting AJAP1 as a putative tumor suppressor. We show that AJAP1 attenuates sprouting angiogenesis by reducing endothelial migration and invasion capacities. Further, we show for the first time that endogenous AJAP1 is associated with the microtubule cytoskeleton. This linkage is independent from cell confluency and stable during angiogenic sprouting in vitro. Our work suggests that AJAP1 is a putative negative regulator of angiogenesis, reducing cell migration and invasion by interfering with the microtubule network. Based on our results and those of other authors, we suggest AJAP1 as a novel tumor suppressor and diagnostic marker.
KW  - AJAP1
KW  - Shrew-1
KW  - Angiogenesis
KW  - Cell migration
KW  - Microtubules
KW  - Endothelial cells
KW  - Cytoskeleton
Y1  - 2017
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/46348
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-463487
SN  - 2046-6390
N1  - Copyright & Usage: © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
VL  - 6
IS  - 6
SP  - 723
EP  - 731
PB  - Company
CY  - Cambridge
ER  -