TY  - JOUR
A1  - Liu, Jing
A1  - Boehme, Philip Patrick
A1  - Zhang, Wenli
A1  - Fu, Jun
A1  - Yumul, Roma
A1  - Mese, Kemal
A1  - Tsoukas, Raphael
A1  - Solanki, Manish
A1  - Kaufmann, Michael
A1  - Lu, Ruirui
A1  - Schmidtko, Achim
A1  - Stewart, A. Francis
A1  - Lieber, André
A1  - Ehrhardt, Anja
T1  - Human adenovirus type 17 from species D transduces endothelial cells and human CD46 is involved in cell entry
T2  - Scientific reports
N2  - More than 70 human adenoviruses with type-dependent pathogenicity have been identified but biological information about the majority of these virus types is scarce. Here we employed multiple sequence alignments and structural information to predict receptor usage for the development of an adenoviral vector with novel biological features. We report the generation of a cloned adenovirus based on human adenovirus type 17 (HAdV17) with high sequence homology to the well characterized human adenovirus type 37 (HAdV37) that causes epidemic keratoconjunctivitis (EKC). Our study revealed that human CD46 (CD46) is involved in cell entry of HAdV17. Moreover, we found that HAdV17 infects endothelial cells (EC) in vitro including primary cells at higher efficiencies compared to the commonly used human adenovirus type 5 (HAdV5). Using a human CD46 transgenic mouse model, we observed that HAdV17 displays a broad tropism in vivo after systemic injection and that it transduces ECs in this mouse model. We conclude that the HAdV17-based vector may provide a novel platform for gene therapy.
KW  - Genetic transduction
KW  - Molecular medicine
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/47178
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-471787
SN  - 2045-2322
N1  - Rights and permissions: Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
VL  - 8
IS  - 1, Art. 13442
SP  - 1
EP  - 14
PB  - Macmillan Publishers Limited, part of Springer Nature
CY  - [London]
ER  -