TY  - JOUR
A1  - Brunkhorst, Robert
A1  - Pfeilschifter, Waltraud
A1  - Patyna, Ralf Sammy
A1  - Büttner, Stefan
A1  - Eckes, Timon Sebastian
A1  - Trautmann, Sandra
A1  - Thomas, Dominique Jeanette
A1  - Pfeilschifter, Josef
A1  - Koch, Alexander
T1  - Preanalytical biases in the measurement of human blood sphingolipids
T2  - International journal of molecular sciences
N2  - Dysregulation of blood sphingolipids is an emerging topic in clinical science. The objective of this study was to determine preanalytical biases that typically occur in clinical and translational studies and that influence measured blood sphingolipid levels. Therefore, we collected blood samples from four healthy male volunteers to investigate the effect of storage conditions (time, temperature, long-term storage, freeze–thaw cycles), blood drawing (venous or arterial sampling, prolonged venous compression), and sample preparation (centrifugation, freezing) on sphingolipid levels measured by LC-MS/MS. Our data show that sphingosine 1-phosphate (S1P) and sphinganine 1-phosphate (SA1P) were upregulated in whole blood samples in a time- and temperature-dependent manner. Increased centrifugation at higher speeds led to lower amounts of S1P and SA1P. All other preanalytical biases did not significantly alter the amounts of S1P and SA1P. Further, in almost all settings, we did not detect differences in (dihydro)ceramide levels. In summary, besides time-, temperature-, and centrifugation-dependent changes in S1P and SA1P levels, sphingolipids in blood remained stable under practically relevant preanalytical conditions.
KW  - sphingolipids
KW  - sphingosine 1-phosphate
KW  - sphinganine 1-phosphate
KW  - ceramides
KW  - dihydroceramides
KW  - blood
Y1  - 2018
UR  - http://publikationen.ub.uni-frankfurt.de/frontdoor/index/index/docId/47215
UR  - https://nbn-resolving.org/urn:nbn:de:hebis:30:3-472150
SN  - 1422-0067
SN  - 1661-6596
N1  - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
VL  - 19
IS  - 5, Art. 1390
SP  - 1
EP  - 15
PB  - Molecular Diversity Preservation International
CY  - Basel
ER  -